FastQCFastQC Report
Mon 6 Feb 2017
SRR2079176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397089
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.035.701951
GGTATCA16950.032.2519381
ATCAACG43250.027.1228753
TCAACGC42550.027.0825654
CAACGCA43200.026.6761845
AACGCAG45700.025.6698726
TATCAAC45100.025.3471832
ACGCAGA53550.021.3265447
CGCAGAG53900.020.9328848
GTACATG63300.019.0761241
TACATGG61800.018.7771222
AGAGTAC52450.018.55785811
ACATGGG66900.017.483023
CGGTTAG1200.001483903217.2387156
CAGAGTA57600.017.138310
GTATAGA8000.016.8672031
GCAGAGT62000.016.3670649
GAGTACT26250.016.3038212
GCACCGA2705.2490577E-815.3348126
GTATATA10900.015.2364271
Produced by FastQC (version 0.11.5)