FastQCFastQC Report
Mon 6 Feb 2017
SRR2079176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397089
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52100.038.7042161
GGTATCA22550.035.7385371
ATCAACG69050.029.089353
TATCAAC69950.028.61642
TCAACGC70350.028.5027484
CAACGCA72250.027.7531955
AACGCAG74800.026.8132216
ACGCAGA88000.022.116987
CGCAGAG87800.021.9297088
GAGTACT56300.021.02135812
AGAGTAC85450.020.35136211
TCGCGCA1056.031389E-419.71147750
CAGAGTA91050.019.2891410
GTACATG64750.019.28391
TCGCCCG2002.8891009E-818.97229822
AGTACTT62950.018.8511613
GCAGAGT97050.018.6654649
GTACTTT63650.018.26903314
TACATGG67050.018.0154022
GTTCGCG1150.001111763217.99743748
Produced by FastQC (version 0.11.5)