FastQCFastQC Report
Mon 6 Feb 2017
SRR2079175_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079175_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998826
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40500.028.6134051
GGTATCA21400.028.126471
TCAACGC48050.024.196664
ATCAACG48550.023.8764043
CAACGCA49700.023.6710135
TATCAAC50400.022.9518322
AACGCAG52850.022.325446
GTACATG71400.018.8949661
ACGCAGA60650.018.4865977
CGCAGAG61500.017.951518
TACATGG71700.017.8190962
GTGTAGC16050.017.6733361
TAGCCCT18650.017.2037454
AGAGTAC57850.016.94198611
ACATGGG75000.016.7439923
GTATAGA9750.015.2561021
CAGAGTA68900.015.07636410
GCAGAGT68700.015.07027159
GAGTACT27150.014.87111812
TCGCGCC1400.004149441614.77683260
Produced by FastQC (version 0.11.5)