FastQCFastQC Report
Mon 6 Feb 2017
SRR2079175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998826
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63350.040.551681
GGTATCA26400.037.8495371
TATCAAC90050.028.86232
ATCAACG90450.028.6965053
TCAACGC90500.028.4518154
CAACGCA93900.027.421615
AACGCAG95850.026.83316
ACGCAGA113250.022.0316687
CGCAGAG115200.021.5057228
GAGTACT79600.021.41319512
AGAGTAC111950.020.68073711
AGTACTT84050.020.06987213
CAGAGTA122050.019.42166910
GTACTTT86800.019.35872814
GCAGAGT124950.019.0813629
GTACATG75700.018.7720891
TACATGG76450.018.5559862
ACATGGG79900.017.279573
TACTTTT102350.016.45127515
ACTTTTT107300.016.33519616
Produced by FastQC (version 0.11.5)