FastQCFastQC Report
Mon 6 Feb 2017
SRR2079174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023294
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23120.11426910770258795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.030.403091
GTATCAA44700.029.0321161
TCAACGC54200.024.1252484
ATCAACG54300.024.0808183
CAACGCA55900.023.3915655
TATCAAC55800.023.2142962
AACGCAG57300.022.6394086
ACGCAGA64450.019.6460387
CGCAGAG64600.019.3867938
AGAGTAC57000.018.22571811
CCTATAC4950.018.1217443
GAGTACT30100.017.88623212
TATACTG8750.017.7433785
CTATACT7550.017.364754
CAGAGTA64750.016.89711210
GTACTTT32350.016.42681914
GTACATG57500.016.3702751
GCAGAGT69600.016.2647299
AGTACTT34750.015.98859613
TACATGG55550.015.8567652
Produced by FastQC (version 0.11.5)