FastQCFastQC Report
Mon 6 Feb 2017
SRR2079174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023294
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.1711565397811687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG350.001348934739.4233745
GGTATCA29650.036.9819151
GTATCAA66200.036.8893931
TATCAAC88350.027.2292082
ATCAACG90550.027.0250533
TCAACGC90450.026.9404534
CAACGCA92150.026.3310855
AACGCAG94150.025.666846
ACGCAGA108400.021.9661247
CGCAGAG108200.021.8775278
AGAGTAC100350.021.07873311
GAGTACT76400.020.7761912
CAGAGTA108550.020.05861510
AGTACTT80550.019.87271513
GCAGAGT112800.019.7311339
GTACTTT82300.019.4925514
TTAGTCG1301.18278454E-418.57447439
ACCGTAC950.007827918.1613358
AGTACGC950.007827918.16133510
GTACATG56850.017.7667181
Produced by FastQC (version 0.11.5)