FastQCFastQC Report
Mon 6 Feb 2017
SRR2079173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519173
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.033.6107981
GGTATCA17000.031.142181
ATCAACG40000.027.5148753
TCAACGC39500.027.3390924
CAACGCA39850.026.839245
TATCAAC41550.026.4274522
AACGCAG43200.024.6780916
GTACATG56550.021.171421
ACGCAGA49950.020.9287827
TACATGG57550.020.0401232
CGCAGAG51450.019.9174528
AGAGTAC50100.018.18724611
GTATACG1150.001110418118.0007343
ACATGGG62150.017.6531753
CAGAGTA55000.016.94344910
GCAGAGT56950.016.6057159
GTATAGC8400.016.0654091
CATGGGG42750.015.011144
GTATATG10100.014.7317251
GAGTACT25550.014.45274912
Produced by FastQC (version 0.11.5)