FastQCFastQC Report
Mon 6 Feb 2017
SRR2079173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519173
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50600.039.5088841
GGTATCA22200.036.7685431
ATCAACG73800.027.732133
TATCAAC72700.027.629532
TCAACGC75100.027.160174
CAACGCA76850.026.5416895
AACGCAG80350.025.3056726
ACGCAGA94900.020.911157
CGCAGAG96800.020.3184038
GTACATG57850.019.6104661
AGAGTAC95550.019.32026711
TACATGG56900.019.1672482
GAGTACT71000.019.148212
GTACTAG3800.019.1140671
CAGAGTA101250.018.33484810
GCAGAGT103850.018.10849
AGTACTT75200.018.02821413
GTACTTT76750.017.76036514
ACATGGG60700.017.6261713
ACCGTCG1250.00193935716.5627068
Produced by FastQC (version 0.11.5)