FastQCFastQC Report
Mon 6 Feb 2017
SRR2079172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844890
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51100.032.4304661
ATCAACG62400.026.5930253
TCAACGC62450.026.4612484
GGTATCA24900.026.3993281
CAACGCA65350.025.1818525
TATCAAC66050.024.991822
AACGCAG67650.024.274716
GTACATG83650.021.0932181
ACGCAGA77800.020.6188937
TACATGG83250.020.5750182
AGAGTAC67100.020.4715711
CGCAGAG79000.020.0440378
ACATGGG88300.019.1444383
GAGTACT33300.018.86230912
CAGAGTA78950.018.09834110
GCAGAGT82600.018.0092439
CATGGGG48250.016.802654
GTACTTT37150.016.72114814
AGTACTT38000.016.7109813
ATACCGT2502.039451E-816.559846
Produced by FastQC (version 0.11.5)