FastQCFastQC Report
Mon 6 Feb 2017
SRR2079172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844890
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74750.037.56441
GGTATCA31950.032.9029121
TATCAAC101000.027.5762522
ATCAACG102850.027.4157943
TCAACGC103550.027.1304764
CAACGCA107050.026.1789635
AACGCAG109950.025.3664866
GAGTACT82850.021.02951412
ACGCAGA131450.020.8965457
CGCAGAG133900.020.355278
AGAGTAC122000.020.30462611
TACATGG87500.020.2739412
AGTACTT86950.020.19377513
GTACATG90950.020.151381
GTACTTT89450.019.51849714
TAGGTCG900.00573792319.16581767
CAGAGTA133500.019.04656210
TACCGTC1651.8261435E-618.8226417
GCAGAGT139800.018.6324589
ACATGGG93000.018.6296143
Produced by FastQC (version 0.11.5)