FastQCFastQC Report
Mon 6 Feb 2017
SRR2079171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633939
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41550.032.731041
GGTATCA22250.026.9063241
TCAACGC50950.026.4775144
ATCAACG52700.025.663753
CAACGCA53350.025.2867495
TATCAAC53850.025.1399152
AACGCAG56850.023.4871946
ACGCAGA64800.020.0183187
GTACATG86350.019.717011
CGCAGAG66000.019.5506138
TACATGG85050.019.0441672
GCGTAAT1150.001088437618.0547581
ACATGGG92000.017.6260383
AGAGTAC66200.017.2576711
GTATACG1452.662038E-416.7058411
CAGAGTA73050.015.97013710
GCAGAGT77700.015.5036699
TATACTG11050.015.2997535
CATGGGG64350.014.8516894
GTGTAGC16950.014.4952921
Produced by FastQC (version 0.11.5)