FastQCFastQC Report
Mon 6 Feb 2017
SRR2079171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633939
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61100.039.1742671
GGTATCA25450.038.7340621
TCAACGC86350.027.9390144
ATCAACG86500.027.8506663
TATCAAC87250.027.3739152
CAACGCA88500.026.9482785
CGCGTAA658.665839E-426.53577265
AACGCAG94550.025.0837886
TATCTCG3750.022.99672136
ACGCAGA110150.021.2738297
CGCAGAG112700.020.5441078
GAGTACT70250.019.94198212
AGAGTAC106900.019.88344611
GTACATG85200.019.5678631
CGCTATC4450.019.3792633
TACATGG84950.018.8110872
CAGAGTA115750.018.69111410
GTATAGG6850.018.6830221
AGTACTT77300.018.2079813
GCAGAGT121400.018.077039
Produced by FastQC (version 0.11.5)