FastQCFastQC Report
Mon 6 Feb 2017
SRR2079170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753209
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37600.034.9663431
GGTATCA18600.031.622081
TCAACGC47350.028.1237374
ATCAACG47600.028.0485083
CAACGCA48400.027.4423355
TATCAAC49750.026.1681162
AACGCAG51400.026.0419986
ACGCAGA59500.021.7426347
CGCAGAG61550.020.8506988
GTACATG74250.020.5492781
TACATGG73550.019.7192692
AGAGTAC57550.019.42957311
ACATGGG77350.018.8662573
CAGAGTA63700.017.71625310
GAGTACT27050.017.35016612
GCAGAGT69250.017.1931329
GTATAGC7300.017.0621621
GTGGTAT11900.016.8630261
GTACTTT29650.016.17760514
CATGGGG54950.016.0095164
Produced by FastQC (version 0.11.5)