FastQCFastQC Report
Mon 6 Feb 2017
SRR2079170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753209
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49100.042.189871
GGTATCA19400.037.4352261
TCAACGC72450.028.9619334
ATCAACG72450.028.9142973
TATCAAC73000.028.60192
CAACGCA73750.028.3578285
AACGCAG77500.026.8579376
TTACGCG658.667384E-426.53468143
ACGCAGA92250.022.2581627
GTACGTA1403.774967E-722.18145811
GAGTACT57200.021.65578712
CGCAGAG94550.021.5679768
AGAGTAC91350.020.81223111
AGTACTT62000.020.1970513
GTACATG74500.020.0535071
CAGAGTA99000.019.37827710
TACATGG76250.019.145332
GCAGAGT102200.018.9403299
GTACTTT65050.018.93704614
ACATGGG79750.017.8723533
Produced by FastQC (version 0.11.5)