FastQCFastQC Report
Mon 6 Feb 2017
SRR2079169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791290
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37000.034.8633541
GGTATCA17400.031.8004171
TCAACGC44600.027.5400054
ATCAACG45150.027.4337773
CAACGCA45450.026.873615
TATCAAC47600.026.1171672
AACGCAG49450.024.6998126
ACGCAGA56750.021.2782297
CGCAGAG57750.020.5513218
GTACATG65050.018.394621
AGAGTAC58750.018.09615711
GTATACG1552.2019614E-517.8492661
TACATGG63950.017.2798462
CAGAGTA62900.017.12172510
GCAGAGT66250.016.7248679
GTGTAGC16250.016.5998151
ACATGGG67250.016.2122653
GAGTACT31150.015.40171612
GTACTTT32600.014.71561514
GTAGCCC12950.014.6535143
Produced by FastQC (version 0.11.5)