FastQCFastQC Report
Mon 6 Feb 2017
SRR2079169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791290
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.039.974491
GTATCAA53200.039.9112741
TATCAAC74000.028.1684992
ATCAACG75000.028.0690063
TCAACGC75100.028.0316334
CAACGCA77200.027.1797075
AACGCAG80650.025.9351656
ACGCAGA94300.021.6273427
CGCAGAG95500.021.1707388
AGAGTAC94200.019.08152211
GAGTACT66100.018.8944112
GTACATG64250.018.8379551
CAGAGTA98950.018.514210
TACATGG62900.018.4900362
GCAGAGT102250.018.2544149
AGTACTT69650.017.78022613
ACATGGG63500.017.4457573
GTACTTT71700.017.32463314
GTATAGC9050.016.4308011
CTCGTAT3800.016.3400339
Produced by FastQC (version 0.11.5)