FastQCFastQC Report
Mon 6 Feb 2017
SRR2079168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587403
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29350.032.1548731
TCAACGC33800.026.5383934
GGTATCA15450.026.4025441
TATCAAC34700.026.2630062
ATCAACG34650.025.9869463
CAACGCA35600.025.0996555
AACGCAG36400.024.1688946
GTACATG57850.020.0783561
ACGCAGA43400.019.6339867
TACATGG57150.019.2683452
CGCAGAG44400.018.880988
ACATGGG60750.017.8320543
AGAGTAC43050.017.47642111
GTACCGT2802.5465852E-1017.2499566
GTGTAGC13250.016.958591
GTATAGA7000.016.7908691
CAGAGTA47250.016.50729210
GCAGAGT50500.015.37669
GTATAGG5750.015.0301891
TATACTG9650.015.0155075
Produced by FastQC (version 0.11.5)