FastQCFastQC Report
Mon 6 Feb 2017
SRR2079168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587403
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41850.039.7559551
GGTATCA17750.035.0773471
ATCAACG58500.028.375923
TCAACGC58800.028.172454
TATCAAC59350.027.9695222
CAACGCA61300.026.8545955
AACGCAG64400.025.4571886
ACGCAGA77150.021.1548717
CGCAGAG79050.020.337718
AGAGTAC77300.019.28067611
GAGTACT51000.018.9411312
CTATGCG1501.6952878E-518.3999549
GTACATG58850.018.3971461
CAGAGTA83450.018.2731710
AGTACTT54800.018.00368913
TACATGG59500.017.8066442
GCAGAGT87300.017.7834599
GTACTTT54650.017.74126214
ACATGGG62050.016.7967743
CGTGCGC4000.015.524960510
Produced by FastQC (version 0.11.5)