FastQCFastQC Report
Mon 6 Feb 2017
SRR2079167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057162
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.036.1164251
GTATCAA51650.033.2995761
ATCAACG65950.025.7386653
TCAACGC67050.025.3678594
CAACGCA68850.024.8549795
TATCAAC68150.024.6245772
AACGCAG71000.024.0051426
GTACATG101250.020.131351
ACGCAGA84100.019.8954147
TATACCG1751.4741454E-719.7150385
CGCAGAG85100.019.5001078
TACATGG100850.019.4135742
ACATGGG105350.018.3395713
AGAGTAC81700.017.99497211
TTGCGCG2551.366061E-917.59063518
CAGAGTA88150.017.1872310
GCAGAGT91900.016.7114939
GAGTACT36000.016.58441512
TGCGCGA1250.001949832516.54952852
GTATAGC11300.016.5374281
Produced by FastQC (version 0.11.5)