FastQCFastQC Report
Mon 6 Feb 2017
SRR2079167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057162
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.043.901561
GTATCAA81450.040.7333561
TATCAAC116100.028.245312
ATCAACG118100.028.0884933
TCAACGC119750.027.7591194
CAACGCA122200.027.0048435
AACGCAG128000.025.6513826
ACGCAGA147550.021.8235957
CGCAGAG148850.021.5356678
AGAGTAC146350.019.96953611
GAGTACT99050.019.71692712
GTACATG101250.019.6127991
TACATGG100900.019.465972
GCGTATC1601.3276076E-619.4601081
CAGAGTA153450.019.42782210
GCAGAGT157050.019.2021889
TTAGTCG2002.8911018E-818.97164339
GTACTTT105200.018.69485914
AGTACTT106050.018.50915113
ACATGGG107150.017.6217923
Produced by FastQC (version 0.11.5)