FastQCFastQC Report
Mon 6 Feb 2017
SRR2079166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979831
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.034.4147071
GTATCAA38250.032.2885631
ATCAACG47950.025.2533133
TCAACGC47950.025.109424
TATCAAC48350.024.781872
CAACGCA48900.024.6921585
AACGCAG52850.023.173056
GTACATG77000.020.3076481
TACATGG77700.019.9093952
ACGCAGA62350.018.6463267
ACATGGG83950.018.0402973
CGCAGAG65500.017.6442578
AGAGTAC60100.016.93978311
GTGGTAT12300.016.5942971
CAGAGTA65000.015.98134910
CATGGGG53450.015.554974
GTATAGG7850.015.4244741
GCAGAGT70800.015.1598449
GTGTAGC12050.014.6418211
GTAGCCC10950.014.4925093
Produced by FastQC (version 0.11.5)