FastQCFastQC Report
Mon 6 Feb 2017
SRR2079166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979831
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.042.5225941
GTATCAA57650.040.0253831
TATCAAC83250.027.9856662
ATCAACG85750.027.3307633
TCAACGC85850.027.1783144
CAACGCA89400.025.9446545
AACGCAG93200.024.9663436
GTACATG76200.020.793111
ACGCAGA111700.020.6365627
CGCAGAG113700.020.0891048
TACATGG78550.019.9931562
GAGTACT75600.019.7619212
AGAGTAC107700.019.35014711
AGTACTT78950.018.91925613
ACATGGG82900.018.4860693
CAGAGTA114450.018.35965710
GTACTTT82500.018.1094114
GCAGAGT120850.017.8727239
TACTTTT90900.016.47416715
TATACTG8400.016.4360285
Produced by FastQC (version 0.11.5)