FastQCFastQC Report
Mon 6 Feb 2017
SRR2079165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461791
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40450.037.530051
GGTATCA17750.036.236631
TCAACGC52300.028.8262274
ATCAACG53450.028.5932883
CAACGCA54500.027.9158065
TATCAAC54550.027.4642622
AACGCAG55950.027.069026
ACGCAGA64800.022.1462367
CGCAGAG64900.021.7404778
GTACATG79850.020.7873671
TACATGG79150.020.19312
GTATAAT10500.019.1016921
AGAGTAC63650.019.0873911
GCAGAGT69450.018.6856889
CAGAGTA68200.018.37065310
ACATGGG83850.017.815293
GAGTACT29400.016.66995812
GTATAGG6200.016.174821
CATGGGG58300.015.9772974
GAGTACA58150.015.6400881
Produced by FastQC (version 0.11.5)