FastQCFastQC Report
Mon 6 Feb 2017
SRR2079165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461791
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.12694010173893722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.045.8502161
GTATCAA69150.040.904381
TATCAAC97900.028.623662
ATCAACG102050.027.6625443
TCAACGC101850.027.6490974
CAACGCA103950.026.9245345
AACGCAG108350.025.773216
GTAACGC1101.1553002E-625.08685727
ACGCAGA128900.021.365387
CGCAGAG129700.021.0971268
GAGTACT86550.020.33261112
AGAGTAC125800.020.24257711
CAGAGTA131700.019.70253410
GCAGAGT133400.019.5031859
AGTACTT90650.019.40865313
GTGGTAT10200.019.3233931
GTACTTT91600.019.13709314
GTACATG80950.018.9232441
TACATGG79650.018.58762
ACATGGG83250.017.7009053
Produced by FastQC (version 0.11.5)