FastQCFastQC Report
Mon 6 Feb 2017
SRR2079164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4310833
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50350.035.5305331
GGTATCA26650.029.4740121
TCAACGC65200.027.6730634
ATCAACG66000.027.4421773
TATCAAC66250.026.7897512
CAACGCA67950.026.603885
AACGCAG70900.025.5942676
ACGCAGA81550.021.278317
CGCAGAG83450.020.3811558
GTACATG100150.019.0721111
AGAGTAC81750.018.78610611
TACATGG98700.018.5417272
CAGAGTA90150.017.72473510
ACATGGG105550.017.061433
GCAGAGT95950.016.8691189
GTATTAG11900.016.2836481
CATGGGG76450.014.350034
GAGTACT39850.014.28810812
GTATATA17450.014.2773531
GTGTAGC19000.014.0232211
Produced by FastQC (version 0.11.5)