FastQCFastQC Report
Mon 6 Feb 2017
SRR2079164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4310833
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63600.039.7461781
GGTATCA28200.034.5808031
TATCAAC90800.027.7507652
ATCAACG92250.027.6513273
TCAACGC92050.027.336424
CAACGCA94900.026.4790945
AACGCAG98350.025.4862566
ACGCAGA114800.021.3153217
GTACATG94750.020.6207431
CGCAGAG119750.020.2858838
TACATGG98800.019.1802082
AGAGTAC116850.019.01686111
GAGTACT75100.018.97543712
ACATGGG102350.017.9753463
CAGAGTA126650.017.79056210
GTATACG1402.0473237E-417.2904011
GCAGAGT134300.017.239649
AGTACTT83850.017.15551813
GTACTTT86650.016.6863914
TATCTCG5300.016.27083236
Produced by FastQC (version 0.11.5)