FastQCFastQC Report
Mon 6 Feb 2017
SRR2079163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387211
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43250.031.3601781
GGTATCA22150.028.8985821
ATCAACG49750.026.974313
TCAACGC48750.026.749214
CAACGCA50900.025.4837825
TATCAAC54700.024.429182
AACGCAG53350.024.0548326
ACGCAGA61350.020.5244777
GTACATG72100.020.39541
CGCAGAG60800.020.0868
TACATGG73050.018.8125692
ACATGGG76600.017.6993623
AGAGTAC60800.017.6550411
CAGAGTA65600.016.62628410
GTGGTAT12700.016.618991
GCGCGAT1250.001935907816.56735411
GCAGAGT71400.016.0972449
TCGATCG1503.532403E-416.09716241
CGTCGTT1851.01966885E-414.9233446
CATGGGG49200.014.7247524
Produced by FastQC (version 0.11.5)