FastQCFastQC Report
Mon 6 Feb 2017
SRR2079163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387211
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61750.038.5319861
GGTATCA26250.036.0986671
ATCAACG83100.028.4884283
TCAACGC84000.028.0599464
TATCAAC85400.027.6807652
CAACGCA87050.026.8785785
AACGCAG90700.025.840696
ACGCAGA105650.021.8202047
CGCAGAG106600.021.5578148
GAGTACT74600.020.81366312
AGAGTAC104500.020.24039611
CAGAGTA108800.019.59902410
GTATACG1952.0989319E-819.5086841
AGTACTT80400.019.35052513
GCAGAGT116750.018.826259
GTACTTT82450.018.78933114
GTACATG73750.018.4289461
TACATGG72400.018.2083322
TATGTCG1351.5745754E-417.8940935
ACATGGG74900.016.863383
Produced by FastQC (version 0.11.5)