FastQCFastQC Report
Mon 6 Feb 2017
SRR2079162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156504
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36900.035.3430941
GGTATCA18050.028.9393881
TCAACGC45250.028.0542624
CAACGCA46450.027.3299355
ATCAACG47400.027.2911953
TATCAAC48300.026.5949552
AACGCAG50750.025.286196
ACGCAGA59500.021.0458347
CGCAGAG61850.019.9682718
AGAGTAC60150.018.0704311
GTACATG69000.017.5973641
TACATGG69200.017.3151722
TACCGTA1804.2048014E-617.2483087
CAGAGTA66400.016.68132210
GAGTACT31600.016.59672712
ACATGGG71000.016.3734873
GCAGAGT70100.016.293359
ACCGTAT2452.923989E-715.4890138
GTGTAGC14150.014.6684571
AGTACTT36850.014.51309913
Produced by FastQC (version 0.11.5)