FastQCFastQC Report
Mon 6 Feb 2017
SRR2079162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156504
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53850.038.862681
GGTATCA22500.034.1297841
ATCAACG74650.028.3410033
TATCAAC73750.028.2188852
TCAACGC75200.027.9960374
CAACGCA79200.026.7128285
AACGCAG81750.025.6319646
ACGCAGA96300.021.3949837
CGCAGAG97600.020.7878158
AGAGTAC93850.019.77982511
GAGTACT64800.019.38210112
TAGCGCG900.005723975619.173975
CAGAGTA99900.018.68556410
AGTACTT69750.018.15097413
GCAGAGT105250.018.0635859
GTACATG68200.017.7519761
GTACTTT73600.017.25274514
TACATGG68900.017.031162
ACATGGG74200.016.2797873
CGATCGT1959.005769E-615.92056642
Produced by FastQC (version 0.11.5)