FastQCFastQC Report
Mon 6 Feb 2017
SRR2079161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568120
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.034.4629941
GGTATCA16000.031.1187211
TCAACGC38500.026.7066864
ATCAACG38900.026.2546713
TATCAAC38550.026.2439582
CAACGCA39550.025.9104185
AACGCAG42800.024.2653876
ACGCAGA48750.020.9490647
CGCAGAG51050.019.5332748
GTACATG67200.019.243391
ATACCGT1651.828319E-618.8201476
TACATGG65400.018.5845052
ACATGGG68950.017.814773
AGAGTAC49650.016.89288311
CAGAGTA53250.016.72310410
GCAGAGT57200.016.6541089
CGAATCG1452.7295836E-416.6507537
CGTGCCG3058.54925E-1015.84327610
GTCCTAG8750.015.4111781
GAGTACT20350.015.09414512
Produced by FastQC (version 0.11.5)