FastQCFastQC Report
Mon 6 Feb 2017
SRR2079161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568120
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.040.0766031
GTATCAA50400.039.119561
TATCAAC71600.027.4776612
ATCAACG73900.026.622473
TCAACGC74150.026.579264
CAACGCA75950.025.722115
AACGCAG78050.024.9911696
ACGCAGA93500.020.4498797
GTACATG62900.020.0720561
GAGTACT64950.019.86934312
CGCAGAG95700.019.79528
TGCGATA1056.02932E-419.7125613
AGTACTT67600.018.88148513
GTACTTT69050.018.68955614
TACATGG65000.018.6385332
AGAGTAC93750.018.62388611
CAGAGTA98400.018.0944610
GCAGAGT101300.017.746779
ACATGGG67900.017.639153
TACTTTT76100.017.04914915
Produced by FastQC (version 0.11.5)