FastQCFastQC Report
Mon 6 Feb 2017
SRR2079160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717768
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31050.032.980221
GGTATCA17600.028.3056561
TCAACGC39400.025.6553544
ATCAACG39550.025.5580483
CAACGCA41000.024.6546235
TATCAAC41450.024.4071772
AACGCAG42700.023.8346466
GTACATG60500.019.8997341
ACGCAGA51350.019.6852867
CGCAGAG51650.019.170538
TACATGG62700.018.558242
GAGTACG2255.355105E-918.4510961
TATACCG1702.4349974E-618.2645155
CGTCGTA950.00781765718.16573110
ACATGGG65750.017.839793
GTATAGC8550.017.8036881
AGAGTAC48900.017.01040611
GTGGTAT9650.016.4912621
CAGAGTA54550.016.2609110
GCAGAGT58150.016.085489
Produced by FastQC (version 0.11.5)