FastQCFastQC Report
Mon 6 Feb 2017
SRR2079160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717768
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46400.039.6480831
GGTATCA19200.036.2012331
ATCAACG66150.027.7558233
TCAACGC66400.027.5473674
TATCAAC66750.027.2478142
CAACGCA67850.027.2129925
AACGCAG71700.025.7102536
ACGCAGA85800.021.1570367
CGCAGAG86750.020.7627778
GAGTACT58300.019.6496112
AGAGTAC86450.019.19837811
GTACATG62450.019.0482251
CAGAGTA92200.018.5250210
AGTACTT62850.018.27727713
GTACTTT64000.018.11555714
GCAGAGT97750.017.790919
TACATGG66800.017.1010932
ACATGGG66850.016.830173
TCTATAC9600.016.1775843
GTATAGC7450.015.7816051
Produced by FastQC (version 0.11.5)