FastQCFastQC Report
Mon 6 Feb 2017
SRR2079159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372695
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.031.8720511
GGTATCA21300.028.255331
TCAACGC44800.026.7210354
ATCAACG45900.026.1558253
CAACGCA46700.025.6342645
TATCAAC46550.025.4438192
AACGCAG49350.024.3276626
ACGCAGA58200.020.3903397
CGCAGAG60850.019.4462268
GTACATG76350.019.2082391
TACATGG74350.018.531042
AGAGTAC58450.018.30483811
ACATGGG79450.017.6726763
CAGAGTA65450.016.61076410
GCAGAGT70000.015.7771229
GTAGCCC17300.015.1554663
CATGGGG54500.014.9388554
GAGTACA56200.014.4015841
CGTGCGC4800.014.38061410
GAGTACT29850.014.33661812
Produced by FastQC (version 0.11.5)