FastQCFastQC Report
Mon 6 Feb 2017
SRR2079159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372695
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61450.038.8426631
GGTATCA26550.037.2634741
TATCAAC85600.027.5402432
ATCAACG87950.027.079093
TCAACGC88050.026.8131334
CAACGCA92100.025.7090075
AACGCAG95900.024.7314246
ACGCAGA110800.021.0871667
CGCAGAG112650.020.613428
GAGTACT75950.019.98870712
AGAGTAC107650.019.77565611
AGTACTT79700.018.95769113
CAGAGTA115800.018.47323410
GTACATG79100.018.2802281
GTACTTT83500.018.09977514
GTATAGG5600.017.9139691
GCAGAGT121800.017.8748269
TACATGG80500.017.8368552
TATACTG13100.017.1262745
ACATGGG81100.017.0239353
Produced by FastQC (version 0.11.5)