FastQCFastQC Report
Mon 6 Feb 2017
SRR2079158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694718
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.035.5459181
GTATCAA40100.032.961991
ATCAACG51150.024.955053
TCAACGC52050.024.324714
CAACGCA52900.023.869095
TATCAAC54950.023.4411642
AACGCAG55100.022.665196
TATCGAC800.002884244321.583312
ACGCAGA62800.019.5554837
GTACATG63900.018.8981381
CGCAGAG65350.018.5826458
AGAGTAC59200.017.72244511
TACATGG66950.017.5374472
GTGTAAG8000.017.3007321
GAGTACT29850.017.10995112
CAGAGTA62750.016.99481610
GCAGAGT67550.015.991269
ACATGGG71450.015.7887563
GTATTAC3950.015.76775551
GTACTTT34700.015.31406214
Produced by FastQC (version 0.11.5)