FastQCFastQC Report
Mon 6 Feb 2017
SRR2079158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694718
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.12116295656911039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.042.7454341
GTATCAA61150.039.498291
ATCAACG87900.027.6417033
TATCAAC87400.027.444442
TCAACGC89200.027.0840874
CAACGCA90800.026.4547965
AACGCAG94850.025.3308456
CGATCGT1752.419256E-1023.6536742
ACGCAGA109900.021.6678737
GTACATG66800.021.6012541
CGCAGAG114050.020.8162068
GAGTACT79600.020.67786612
TACATGG69900.020.589222
AGAGTAC108300.019.913711
ACATGGG73700.019.621293
AGTACTT84500.019.47409413
CAGAGTA112900.019.46910310
GCAGAGT116700.019.1012619
GTACTTT88800.018.72777214
CGTATGC4850.018.49212841
Produced by FastQC (version 0.11.5)