FastQCFastQC Report
Mon 6 Feb 2017
SRR2079157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670451
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37450.036.5733031
GGTATCA17900.034.9740831
ATCAACG48000.028.1745953
TCAACGC48250.028.0286124
CAACGCA49300.027.5721285
TATCAAC50550.026.3616142
AACGCAG51400.026.2442786
ACGCAGA58350.022.4079997
CGCAGAG61950.020.8834748
GTACATG72200.020.6950911
AGAGTAC55650.020.52607311
TACATGG72900.019.7949582
CAGAGTA60050.019.13701810
ACATGGG75950.018.6692523
GAGTACT25250.018.03977812
GCAGAGT66700.017.95349
GCGTATC1452.6718946E-416.6974751
CATGGGG47550.016.6874564
AGTACTT30950.015.49731713
TAATACT12700.015.212384
Produced by FastQC (version 0.11.5)