FastQCFastQC Report
Mon 6 Feb 2017
SRR2079157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670451
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54250.040.750041
GGTATCA21800.038.087431
ATCAACG79350.027.624893
TCAACGC80250.027.2290464
TATCAAC80600.026.9823192
CAACGCA83800.025.9519675
AACGCAG87550.024.5309166
ACGCAGA102600.020.5867777
GAGTACT65550.020.21291212
CGCAGAG104550.020.1978868
TATACCG1559.998475E-720.0440085
TACGTCG1056.0021E-419.725855
GTACATG75450.019.4416681
AGAGTAC100100.018.9927511
TACATGG75400.018.8167342
AGTACTT71100.018.72725913
GTACTTT72550.018.3095414
CAGAGTA105800.018.09996210
ACATGGG80400.018.0758933
GCAGAGT109250.017.9705459
Produced by FastQC (version 0.11.5)