FastQCFastQC Report
Mon 6 Feb 2017
SRR2079156_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079156_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919810
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33600.034.8976021
ATCAACG40550.028.4993023
ATGCGCG854.4218996E-628.41944951
GGTATCA17150.028.034051
TCAACGC40250.027.7689484
CAACGCA42550.026.2679215
TATCAAC43350.025.9685692
AACGCAG44400.025.095736
ACGCAGA49950.022.030317
CGCAGAG51850.021.1575858
GTACATG54950.020.7092041
TACATGG55500.019.848022
ACATGGG57050.019.1078453
AGAGTAC51850.018.23668311
GAGTACT29650.018.15668112
CAGAGTA56150.016.96302610
GCAGAGT60150.016.3510539
CGTGCGC3209.822543E-1116.17652710
TCTATAC8100.015.7578523
GTGTAGC14800.015.1910161
Produced by FastQC (version 0.11.5)