FastQCFastQC Report
Mon 6 Feb 2017
SRR2079156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919810
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51200.040.7952271
GGTATCA20850.034.1667371
ATCAACG73550.028.4848613
TCAACGC74800.028.0088464
TATCAAC74100.027.9939612
CAACGCA76600.027.1704395
AACGCAG79200.026.2825326
ACGCAGA90650.022.5746277
GAGTACT66800.022.31382412
CGCAGAG92200.021.8926898
AGAGTAC88350.021.20606211
GTACATG54750.020.3382741
CAGAGTA94050.020.14096510
AGTACTT75950.019.80391513
GTACTTT76800.019.63368614
GCAGAGT98650.019.5865389
TACATGG55350.019.2061862
GCCGATT1702.4383098E-618.26201862
AGTCGCG1200.001476091117.25248518
TACTTTT89600.016.86738415
Produced by FastQC (version 0.11.5)