FastQCFastQC Report
Mon 6 Feb 2017
SRR2079155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847501
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36600.036.7685741
GGTATCA18600.035.1525731
TCAACGC49350.027.2637794
CAACGCA50000.026.7028245
ATCAACG50800.026.6893163
TATCAAC51450.026.0447792
AACGCAG51500.025.9920626
ACGCAGA61350.021.1437427
CGCAGAG63250.020.1274958
GTACATG68800.019.5600241
AGAGTAC60900.019.15236711
TACATGG70050.018.7841322
CAGAGTA67850.017.54657410
GCAGAGT69650.017.5390159
GAGTACT31350.016.8407612
ACATGGG75600.016.3825423
GTACTTT33850.015.90084914
GTATATA10600.015.6654761
AGTACTT34200.015.63861113
GTATAGA8200.015.1878711
Produced by FastQC (version 0.11.5)