FastQCFastQC Report
Mon 6 Feb 2017
SRR2079155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847501
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51700.040.5387841
GGTATCA22000.039.4584161
ATCAACG76650.027.9584713
TATCAAC75050.027.7728352
TCAACGC78550.027.1504024
CAACGCA80050.026.2536645
AACGCAG83500.025.1751216
ACGTCGT1202.4629426E-622.99679223
ACGCAGA99750.020.9307147
CGCAGAG102550.020.0875458
GTACATG70450.019.7839341
AACGTCG1451.2673989E-519.03182822
AGAGTAC98750.018.9387111
GAGTACT64700.018.82602312
CAGAGTA103650.018.27642310
TACATGG73000.018.1054732
GCAGAGT107850.017.9806069
GTATAAC4700.017.660461
TACCGTA2551.364242E-917.5931977
ACCGTAT2751.9645086E-1017.5664318
Produced by FastQC (version 0.11.5)