FastQCFastQC Report
Mon 6 Feb 2017
SRR2079154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717405
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.031.1895891
GTATCAA28750.030.5723321
TCAACGC33650.025.8389454
ATCAACG33600.025.2612673
CAACGCA34600.024.5311725
AACGCAG36250.023.224226
TATCAAC37300.022.7764282
ACGCAGA42400.019.772787
CGCAGAG43450.019.1368568
GTACATG54550.018.6502741
TATACTG10800.017.5710725
TACATGG54500.017.489282
GTACCGT2005.9899503E-717.2515966
AGAGTAC44300.017.21804411
ACATGGG55800.016.633263
GTATAGC9800.016.5960391
TAGCCCT17700.015.98452954
ACCGTGC4800.015.8133828
CAGAGTA49400.015.789803510
GACTGCG3101.0841177E-915.5809417
Produced by FastQC (version 0.11.5)