FastQCFastQC Report
Mon 6 Feb 2017
SRR2079154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717405
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43400.038.9586221
GGTATCA18450.037.106781
ATCAACG62500.027.8318923
TCAACGC62250.027.6110024
TATCAAC62050.027.3106422
CAACGCA65600.026.1483765
AACGCAG66750.025.8069936
ACGCAGA79200.021.0473847
CGCAGAG80900.020.4722218
AGAGTAC80800.018.405111
GTACATG58050.017.9286981
GAGTACT56350.017.81850812
TCGTATG3300.017.77032540
CAGAGTA85500.017.71620410
GCAGAGT88950.017.339399
TAGTCGG1804.2058546E-617.2476740
TACATGG59450.016.8359782
AGTACTT61400.016.79883613
CTATACT8700.016.6618124
GTACTTT62300.016.39426414
Produced by FastQC (version 0.11.5)