FastQCFastQC Report
Mon 6 Feb 2017
SRR2079153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620573
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36000.032.8667371
GGTATCA19100.027.713481
ATCAACG45200.025.8736253
TCAACGC44850.025.8447824
TATCAAC46800.025.0167542
CAACGCA46650.024.8475575
AACGCAG47700.024.3005966
ACGCAGA57200.020.023037
GTACATG68100.019.4573941
CGCAGAG59200.018.9390288
TACATGG66850.018.8051622
AGAGTAC54650.018.68947411
GTATAGT4150.016.6730441
ATACCGT2702.928573E-916.6102286
CAGAGTA62050.016.57181410
GCAGAGT64100.016.4727929
ACATGGG72350.016.4027233
GAGTACT25200.015.74531612
GTATAGG5700.015.1739321
AGTACTT28250.014.77818213
Produced by FastQC (version 0.11.5)