FastQCFastQC Report
Mon 6 Feb 2017
SRR2079153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620573
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.039.2699281
GTATCAA55450.039.234791
GTATACG901.952867E-730.7446611
TATCAAC77450.028.0283182
TCAACGC80250.026.964374
ATCAACG80950.026.944373
CAACGCA82650.026.0978645
AACGCAG86100.025.0158676
ACGCAGA100350.021.1471067
CGCAGAG102000.020.49868
GTACATG70050.019.849071
AGAGTAC98450.019.59060511
TACATGG69100.019.4285032
GAGTACT66750.019.3317912
CAGAGTA104600.018.80160510
GCAGAGT108550.018.371729
AGTACTT69000.018.2482413
GTACTTT71000.017.88269414
ACATGGG75850.017.8360353
TACTTTT78300.016.08327915
Produced by FastQC (version 0.11.5)