FastQCFastQC Report
Mon 6 Feb 2017
SRR2079152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272440
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27600.033.207091
TATATCG703.853071E-529.5709555
GGTATCA12950.028.5764331
ATCAACG32200.027.6424183
TCAACGC32100.027.513584
CAACGCA33750.026.1684725
AACGCAG35200.025.286536
TATCAAC35150.025.0484562
CGCAGAG39600.021.605248
ACGCAGA40200.021.5392787
GTACATG46700.020.6624261
TACATGG46700.019.5927352
AGAGTAC38850.018.56527311
ACATGGG49550.018.3115143
GCAGAGT45800.017.3311589
CAGAGTA43800.017.09746410
GTATAGG4900.016.9398351
TATACTG6650.016.082455
GTATATA10350.016.039651
CCTATAC3900.015.9228243
Produced by FastQC (version 0.11.5)