FastQCFastQC Report
Mon 6 Feb 2017
SRR2079152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272440
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41350.037.399011
GGTATCA18050.035.6503521
ATCAACG54800.028.1552523
TCAACGC55650.027.7252064
TATCAAC56650.026.9311472
CAACGCA57250.026.7091885
AACGCAG60300.025.4205046
TATACCG750.001987930623.0113035
ACGCAGA70500.021.4897527
CGCAGAG72250.020.7745158
AGAGTAC69400.019.3894511
GAGTACT49600.019.0602812
GTACATG47250.018.8906081
CAGAGTA74100.018.39243710
TACATGG49650.017.9363022
GCAGAGT79050.017.8517919
GTACTTT55250.017.23830214
AGTACTT55150.017.20131113
ACATGGG51500.017.1579423
CTATGCG1704.960275E-516.2368379
Produced by FastQC (version 0.11.5)